Upshot of Gentamicin and role of Antioxidant on Spermatogenesis of Albino rats

The process of gametogenesis in the male occurs within the seminiferous tubules of the testes, resulting in the production of sperm. Many factors like Drugs, chemotherapy and toxins can generate harmful effect on spermatogenesis and affect sperm normal production. Gentamycin is a synthetic antibacterial agent belonging to the family of amino glycoside. Antioxidants scavengers of free radicals can ameliorate the severity of the effects of antibiotics. The study was performed on male Albino Wistar rats Animals were obtained from the Animal House, Mamata Medical College, and Khammam, India. The experimental animals were divided into three groups of six rats each as follows. Group I: In Control rats, normal saline was given followed by oil vehicle for 14 days. Group II: Rats received gentamicin for 14 days. Group III: Rats were injected with gentamicin as in group II and concomitantly supplemented with Coenzyme Q10 for 14 days. The testicular tissue antioxidant, Malondialdehyde (MDA) a product of Lipid per oxidation, sperm count, % sperm motility, sperm abnormality, quantitative analysis of spermatogenesis and testicular markers like testosterone, Lactate dehydrogenase (LDH) and Sorbitol dehydrogenase (SDH) were measured in all three groups. In results Compared to the control group, considerable drop in epididymal sperm count was observed in Group I. When this dose of gentamicin was administrated together with Coenzyme Q10 respectively the epididymal sperm count level was appreciably increased in Group III. The % sperm motility was higher in Group III than other two groups. The testicular antioxidant enzyme was significantly lowered in group II than controls and Group III. MDA was significantly elevated in group II than controls and Group III. In conclusion the Antioxidant therapy could help improve the spermatogenesis and % of sperm motility, SOD, LDH, SDH, and testosterone and decreased the sperm abnormalities and MDA.